Measurement of Transcobalamin by ELISA

Measurement of transcobalamin by ELISA.

BACKGROUND Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin. METHODS gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture ...

Measurement of Epsilon Antitoxin in Sheep Sera by ELISA

Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Measurement of aflatoxin M1 by ELISA method in milk samples produced by cattle in farms of Shahre Ghods, Shahriar, Iran

Background/objective: Aflatoxin M1 (AFM1) is a metabolite of the aflatoxin B1 found in the liver of livestock, as a result of feeding livestock with contaminated food. Certain species of Aspergillus are responsible for producing aflatoxin. The present study was performed to evaluate the measurement of AFM1, by ELISA method, in milk samples collected from dairy farms of Shahre Ghods, Shahriar (T...

measurement of epsilon antitoxin in sheep sera by elisa

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